At NCIMB our highly experienced microbiologists have decades of experience of identifying, quantifying and monitoring microbes that can cause problems in industrial or environmental settings.
Total viable counts – bacteria and fungi
Total viable counts give a general indication of numbers of bacteria present in a sample. This technique can be a good starting point for any investigation into suspected microbiological contamination within industrial settings, and can also be used to monitor responses to any treatment subsequently undertaken.
Culture-based enumeration of specific groups of bacteria
If you want information on the numbers of specific groups of bacteria within a sample, rather than an estimate of the total number of viable organisms present, we can use culture-base methods with selective media to estimate numbers. For example, we regularly undertake testing to determine numbers of sulphate reducing bacteria, general heterotrophic bacteria and acid producing general heterotrophic bacteria.
We also supply media testing kits if you would like to undertake this kind of testing yourself. We have an extensive media recipe library, so please contact us for information about any other groups of bacteria you are interested in.
Quantitative polymerase chain reaction – qPCR
If you need to confirm the presence or absence of a group of bacteria in a product, process, effluent or environment, quantify the numbers present, or monitor the population over time, we can use qPCR to achieve this.
qPCR is a molecular biology technique rather than a culture based methodology. In other words, there is no requirement for growth of the microorganisms present in qPCR testing. This means results can be obtained very rapidly, and microbes that do not grow under laboratory conditions will be included in the results.
We can develop customised qPCR assays for specific organisms of interest to individual clients, or undertake assays for a range of commonly requested organisms.
Identification of bacteria and fungi
Total viable counts give a general indication of numbers of microbes and whether an issue you are experiencing may be due to microbial contamination.
Identifying the species present can be a valuable next step in investigating the source of the problem, determining remediation steps and monitoring for recurrence of the same issues in future.
We undertake rapid identification of bacterial and fungal isolates using sequencing-based methods. This approach can identify viable and non-viable organisms as well as those with complex growth requirements and Gram-variable isolates.
Reporting can be tailored to your requirements.
Microbial community analysis
In some situations, analysis of the whole microbial community may be the most appropriate course of action.
Microbial community analysis uses DNA sequencing information to analyse the whole microbial population from a single sample. It identifies the groups of microorganism present to give a comprehensive picture of the microbial ecosystem. At NCIMB we can undertake microbial community analysis to help customers assess soil health, identify the different populations of microorganisms present during bioremediation processes or in industrial effluents.
We offer a flexible service with rapid same or next day options on some identification services.
We can advise you on the most appropriate approach for sampling and testing. Please contact us for more information.