Yeasts and moulds
Fungi can cause serious problems for pharmaceutical manufacturers, and species level identification gives valuable information that can be used when investigating contamination issues or excursions from normal populations.
There are two distinct approaches to genotypic identification of fungi: sequencing of the D2 region of the large subunit ribosomal DNA (D2 LSU), and sequencing of either one or both of the internal transcribed spacer regions between the small and large subunit ribosomal RNA genes (ITS). At NCIMB we combine these techniques to offer a comprehensive service that gives our customers the most reliable species level identification available.
For D2 LSU identification, sequences are analysed against the validated MicroSEQ database. In some cases, however, this only gives a genus level identification. When this occurs we often find a species level identification can be obtained by using the ITS method.
Next generation sequencing
We offer next generation sequencing for microbial community analysis and whole genome sequencing. Whole genome sequencing is the ultimage in organism characterisation. It is a valuable technique for microbiome studies, the discovery of useful properties, searching for antibiotic resistance genes, identifying virulence factors and strain comparison.
Pure cultures can be sent to us for identification as plates, slopes or broths that have been incubated to produce sufficient growth. Please complete a sequencing request form and send with isolates to be identified.
We can also accept mixed cultures and other sample formats, such as samples of water, swabs, slimes or contaminated products, but please contact us to discuss the options available prior to submitting them for identification. Same and next day services may not be available for these sample types.
NCIMB does not accept active cultures of dangerous human pathogens or organisms isolated from clinical or diseased plant materials for identification. We cannot accept isolates known to be greater than ACDP category 2 and ACGM class 1.
Take care to package samples appropriately. Detailed advice on the packaging of isolates is available on request. Please contact us for more information.