Whole Genome Sequencing of Bacteria

Microbial strains can be an essential part of research programmes, included in products such as probiotic supplements, or used as production strains for the manufacture of a diverse range of products.  Whole genome sequencing provides the deepest level of insight into your precious biological resource, helping you to fully understand and protect it. The data generated can be revisited and reanalysed to meet changing requirements.

Bacterial Identification

Monitor for strain drift

Contamination and strain drift can occur in working stocks of production strains, or within research laboratories where strains are repeatedly subcultured during long term research projects. Regularly comparing sequences of working stocks with the sequence of the original isolate or banked stock highlights any indication of drift over time.

Protect intellectual property

Whole genome sequencing can be very important with respect to the industrial use of bacteria. For example, companies may specify the presence of a particular strain within a patented process. In cases where a patent is contested, or infringement suspected, whole genome sequencing allows very closely related strains to be compared and differentiated.

Whole genome sequencing for secondary metobolite prediction and natural product analysis

A high-quality whole-genome sequence enables the ultimate in strain characterisation, and can be a first step in the development of new or improved microbial applications and processes. Whole-genome sequencing allows us to take a deep dive into a strain, and can be used to rapidly screen for the production of industrially or clinically important secondary metabolites. With the latest tools for genome annotation and genome mining, we ensure that our customers get maximum value from their genomic data. We offer whole genome sequencing as service to customers wishing to sequence their own strains, and also work in collaboration with other researchers to explore the potential of our own culture collection.

Whole genome sequencing and characterisation of strains used in the food chain

Whole genome sequencing is a key consideration in the responsible use of microbes as food and animal feed additives, as well as for other industrial applications such as biotherapeutics. Our comprehensive genomics service includes genome sequencing, assembly and annotation, including prediction of antimicrobial resistance genes and virulence factors. We work to ensure regulatory compliance for our customers, and offer a package of services to meet EFSA/FEEDAP guidance for animal feed additives. Once sequenced, the whole genome data is available for any future analysis required Services can be tailored to meet individual customer needs for a range of industry sectors, and we can undertake bioinformatics analysis on existing datasets. We also offer a full range of microbiology tests and analyses to help you determine the functional characteristics of candidate strains and assess their suitability for use in probiotic products and other industrial applications. More information about our services services for animal feed additives and probiotic products can be found here. Find out more about the work we have done to help screen candidate strains for live biotherapeutics development in our case study.

Three-point strain health check service

NCIMB offers a whole-genome sequencing-based three-point strain health check service that is applicable to microbial strains used in industrial biotechnology, synthetic biology, fermentation systems such as anaerobic digesters and food and drink manufacturing - in fact, any application where your process relies on microbial products. The three elements of our strain health check are "Identify", "Monitor" and "Comply" - identify and assess strain functionality; monitor for strains drift and comply with regulations and mitigate risk. The service includes an initial consultation to determine and agree the required work scope after which the sample can be shipped to us. We will then carry out the sample preparation and DNA extraction prior to whole genome sequencing. This is followed by bioinformatics analysis, comparative genomics (either to compare working and master stocks, or to help select the most suitable strain for a particular purpose), reporting and a final consultation.

Easy sample submission

You can submit DNA or pure cultures for whole genome sequencing. If you don't have the time or facilities to pre-prepare samples, we can accept mixed cultures, lyophilised end products, and other sample types such as water, slimes or contaminated products for DNA extraction prior to sequencing. Please contact us for more information about our services.

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