Next generation sequencing for analysing bacterial communities – an introduction

Next generation sequencing has transformed understanding of bacterial communities, and in particular the gut microbiome. Consequently, it is becoming increasingly popular in a variety of industry sectors as people begin to understand how they can use the data generated. However, it can be difficult to get to grips with the terminology and processes involved in next generation sequencing, so in this blog NCIMB’s microbiome services manager Julie MacKinnon takes a closer look at the process for analysis of mixed communities of bacteria.  The application of next generation sequencing techniques to …

Metagenomics, metataxonomics or 16S amplicon sequencing? An introduction to microbiome analysis.

by Julie MacKinnon, Microbiome Services Manager High throughput sequencing of the 16S ribosomal RNA gene has become an increasingly popular analysis in recent years. One of the most high-profile applications of this technology has been in analysing and understanding the human gut microbiome and this is a service that we provide at NCIMB to support our customers’ research and development work. Analysing stool samples may not sound like the most pleasant occupation, but the research being undertaken in this area, for example, in understanding the relationship between microbiome, nutrition and …